Name: Z-IETD-FMK
Brand: AAT Bioquest
SKU: 13305
Price: 108 USD
Availability: InStock

Z-IETD-FMK

Z-IETD-FMK is a cell-permeable, irreversible caspase-8 inhibitor. It is widely used in cell apoptosis studies. Caspase-8 is a member of the cysteine proteases, which are implicated in apoptosis and cytokine processing. Like all caspases, caspase-8 is synthesized as an inactive single polypeptide chain zymogen procaspase and is activated by proteolytic cleavage, through either autoactivation after recruitment into a multimeric complex or trans-cleavage by other caspases. Thus, ligand binding-induced trimerization of death receptors results in recruitment of the receptor-specific adapter protein Fas-associated death domain (FADD), which then recruits caspase-8. Activated caspase-8 is known to propagate the apoptotic signal either by directly cleaving and activating downstream caspases or by cleaving the BH3 Bcl2-interacting protein, which leads to the release of cytochrome c from mitochondria, triggering activation of caspase-9 in a complex with dATP and Apaf-1. Activated caspase-9 then activates further "downstream caspases," including caspase-8. Knockout data indicate that caspase-8 is required for killing induced by the death receptors Fas, tumor necrosis factor receptor 1, and death receptor 3. Moreover, caspase-8-/- mice die in utero as a result of defective development of heart muscle and display fewer hematopoietic progenitor cells, suggesting that the FADD/caspase-8 pathway is absolutely required for growth and development of specific cell types.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13305	1 mg	Price

References

View all 27 references: Citation Explorer

Role of caspases in CD95-induced biphasic activation of acid sphingomyelinase.

Authors:

Stephan, Mario and Edelmann, Bärbel and Winoto-Morbach, Supandi and Janssen, Ottmar and Bertsch, Uwe and Perrotta, Cristiana and Schütze, Stefan and Fritsch, Jürgen

Journal:

Oncotarget (2017): 20067-20085

Caspase-8 inhibition represses initial human monocyte activation in septic shock model.

Authors:

Oliva-Martin, Maria Jose and Sanchez-Abarca, Luis Ignacio and Rodhe, Johanna and Carrillo-Jimenez, Alejandro and Vlachos, Pinelopi and Herrera, Antonio Jose and Garcia-Quintanilla, Albert and Caballero-Velazquez, Teresa and Perez-Simon, Jose Antonio and Joseph, Bertrand and Venero, Jose Luis

Journal:

Oncotarget (2016): 37456-37470

Caspase blockade induces RIP3-mediated programmed necrosis in Toll-like receptor-activated microglia.

Authors:

Kim, S J and Li, Jianrong

Journal:

Cell death & disease (2013): e716

Cytokeratin 18 expression inhibits cytokine-induced death of cervical cancer cells.

Authors:

Sullivan, Brian T and Cherry, Jessica A and Sakamoto, Hideo and Henkes, Luiz E and Townson, David H and Rueda, Bo R

Journal:

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (2010): 1474-81

Non-canonical role for the TRAIL receptor DR5/FADD/caspase pathway in the regulation of MyoD expression and skeletal myoblast differentiation.

Authors:

Freer-Prokop, Margot and O'Flaherty, John and Ross, Jason A and Weyman, Crystal M

Journal:

Differentiation; research in biological diversity (2009): 205-12

Physical properties

Molecular weight	654.69
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 29, 2025