Z-VAD-FMK
Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethylketone) is a cell-permeable, irreversible pan caspase inhibitor that binds to the catalytic site of caspase proteases and can inhibit induction of apoptosis. For inhibition of apoptosis, Z-VAD-FMK should be added at the same time that apoptosis is induced. The peptide is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability. Fluoromethyl ketone (FMK)-derivatized peptides act as effective irreversible inhibitors with no added cytotoxic effects.
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Note Store the unused Z-VAD-FMK stock solution at -20 °C in single use aliquots.
Z-VAD-FMK stock solution
Add appropriate amount of DMSO into Z-VAD-FMK to make 2-5 mM Z-VAD-FMK stock solution. Note Store the unused Z-VAD-FMK stock solution at -20 °C in single use aliquots.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used as a guideline and should be optimized according to your needs.
- Treat your samples as desired to induce apoptosis.
- Add Z-VAD-FMK along with the treatment of your choice.
Note We recommend using 10-100 µM of final concentration ofZ-VAD-FMK to inhibit caspases such as 50 µM for caspase-8.
Note Optimal time and concentration for incubation needs to be determined experimentally. - Stain samples as you desired.
- Monitor the fluorescence intensity with a proper instrument.
References
View all 8 references: Citation Explorer
Identification of active caspases using affinity-based probes.
Authors: McStay, Gavin P and Green, Douglas R
Journal: Cold Spring Harbor protocols (2014): 856-60
Authors: McStay, Gavin P and Green, Douglas R
Journal: Cold Spring Harbor protocols (2014): 856-60
Induction of apoptosis in human renal cell carcinoma cells by vitamin E succinate in caspase-independent manner.
Authors: Wu, Xiu-Xian and Kakehi, Yoshiyuki and Jin, Xing-Hua and Inui, Masashi and Sugimoto, Mikio
Journal: Urology (2009): 193-9
Authors: Wu, Xiu-Xian and Kakehi, Yoshiyuki and Jin, Xing-Hua and Inui, Masashi and Sugimoto, Mikio
Journal: Urology (2009): 193-9
Induction of apoptosis in renal tubular cells by histone deacetylase inhibitors, a family of anticancer agents.
Authors: Dong, Guie and Wang, Lysa and Wang, Cong-Yi and Yang, Tianxin and Kumar, M Vijay and Dong, Zheng
Journal: The Journal of pharmacology and experimental therapeutics (2008): 978-84
Authors: Dong, Guie and Wang, Lysa and Wang, Cong-Yi and Yang, Tianxin and Kumar, M Vijay and Dong, Zheng
Journal: The Journal of pharmacology and experimental therapeutics (2008): 978-84
Metformin induces apoptosis of pancreatic cancer cells.
Authors: Wang, Luo-Wei and Li, Zhao-Shen and Zou, Duo-Wu and Jin, Zhen-Dong and Gao, Jun and Xu, Guo-Ming
Journal: World journal of gastroenterology (2008): 7192-8
Authors: Wang, Luo-Wei and Li, Zhao-Shen and Zou, Duo-Wu and Jin, Zhen-Dong and Gao, Jun and Xu, Guo-Ming
Journal: World journal of gastroenterology (2008): 7192-8
Apoptosis of gastric cancer cell line MKN45 by photodynamic treatment with photofrin.
Authors: Takahira, Kenichiro and Sano, Munetaka and Arai, Hajime and Hanai, Hiroyuki
Journal: Lasers in medical science (2004): 89-94
Authors: Takahira, Kenichiro and Sano, Munetaka and Arai, Hajime and Hanai, Hiroyuki
Journal: Lasers in medical science (2004): 89-94
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