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iFluor® Dye Selection Guide
AAT Bioquest iFluor® dyes are a series of highly water-soluble fluorescent dyes for labeling biomolecules, such as proteins and antibodies. Available in a variety of excitation-emission profiles, spanning the UV-visible to near-infrared spectrum, there is an iFluor® dye to match any instrument set-up and many combinations for multiplex detection. Conjugates made with iFluor® dyes exhibit superior brightness and photostability, outperforming most Alexa Fluor® conjugates and other spectrally similar conjugates.
Benefits of iFluor® Dyes and Conjugates
- Brightness: iFluor® conjugates exhibit more intense fluorescence than other spectrally similar conjugates (e.g. Alexa Fluor® dyes, fluorescein dyes, cyanine dyes, etc.)
- Photostability: iFluor® conjugates are more photostable than spectrally similar conjugates allowing for more time to capture images.
- Water solubility: iFluor® reactive dyes are highly water soluble. Conjugation can be performed without organic solvents resulting in conjugates that are resistant to precipitation during storage.
- pH insensitivity: iFluor® dyes are robust and highly fluorescent across a broad pH range.
- Instrument compatibility: Absorption spectra of iFluor® dyes are matched to the principal output wavelengths of common excitation sources.
- Color selection: iFluor® dyes are available in several distinct colors, ranging from violet (350 nm) to infrared (860 nm), maximizing choice and flexibility in multicolor analysis.
Fig. 1
Image of HeLa cells stained with mouse anti-tubulin followed with iFluor® 594 goat anti-mouse IgG. Actin filaments were stained with Phalloidin-iFluor® 488 Conjugate and nuclei were stained with DAPI.
iFluor® Dyes Spectral Properties
iFluor® Dye Selection Guide
Document: 02.0125.190828r1
Last updated Fri Oct 03 2025
iFluor® Dye Selection Guide