by Jinfang Liao, Jixiang Liu, Qin Zhao, Ruogu Peng, Yunting Xi, Zhenjun Diwu, Zhen Luo
Flow cytometry analysis can provide important spatial and temporal information of multiple biomarkers in individual cells simultaneously. However, most of the flow cytometry analysis are still limited to total DNA and protein expression in cells. It is well known that detecting and imaging RNA molecules in live cells is extremely important for a wide variety of molecular biology applications. To measure RNA level in cells, commonly used techniques such as polymerase chain reaction (PCR), microarrays, and fluorescence in situ hybridization (FISH) are still limited to lysed and fixed cells. The major challenge in analyzing RNA in live cells is the interference caused by DNA. To address these difficulties, we have developed
StrandBrite™ RNA Green, an excellent RNA-selective probe that generates significantly enhanced green fluorescence upon binding to RNA. It has been successfully used for flow cytometric analysis of live cells.
Imaging RNA in Live and Fixed Cells
StrandBrite RNA Green vs. SYTO® RNASelect
Flow Cytometric Analysis of RNA in Cells
Selectivity Test of StrandBrite RNA Green