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Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Posted June 12, 2019
Answer
Yes, you can. By using a low affinity fluorescent calcium indicator, e.g., Calbryte-520 AM or Fluo-4 AM, and a mitochondria membrane potential marker such as TMRM, you can simultaneously monitor mitochondria calcium flux and membrane potential changes at the same time.
For a detailed protocol, please refer to the article below.
Additional resources
Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*
TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*