Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Posted June 12, 2019
Yes, you can. By using a low affinity fluorescent calcium indicator, e.g., Calbryte-520 AM or Fluo-4 AM, and a mitochondria membrane potential marker such as TMRM, you can simultaneously monitor mitochondria calcium flux and membrane potential changes at the same time.
For a detailed protocol, please refer to the article below.