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Can I use azide in ELISA buffers?
Posted October 8, 2021
Enzyme-Linked Immunosorbent Assay (ELISA)Horseradish Peroxidase (HRP) and Poly-HRPEnzymes
Answer
No, you cannot use azide in ELISA buffers. Azide is an irreversible inhibitor of horseradish peroxidase (HRP). It inactivates HRP enzyme. 
Additional resources
Enzyme-Linked Immunosorbent Assay (ELISA)Stayright™ Purple HRP Staining Kit

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