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How is ASC oligomerization detected?

Posted October 13, 2021


Answer

ASC oligomerization is detected by Western Blotting. Inflammasome assembly results in the formation of a large intracellular protein scaffold driven by the oligomerization of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC). Following inflammasome activation, ASC polymerizes to form the ASC speck, a large singular structure that is essential for recruitment of caspase-1 and its inflammatory activity. Because of the relatively large size of these structures, ASC specks can be easily detected by microscopy.

Additional resources

Detection of ASC Oligomerization by Western Blotting

Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit *Triple Fluorescence Colors*

Apoptosis and Necrosis