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What components are needed in cell culture media?
Posted July 22, 2020
Answer
The components generally required for the cell culture media are as follows:
- Carbon source: Glucose is often used as the carbon source to provide energy for the cells.
- Amino acids: They are the building blocks of protein.
- Vitamins: They can promote cell survival and growth.
- Balanced salt solution: It is used to maintain the optimum osmotic pressure within the cells and provide essential metal ions to serve as cofactors for enzymatic reactions.
- Phenol red dye: It is used to indicate pH change. The color of phenol red at neutral pH (7-7.4) is orange/red, which changes to yellow at acidic pH and purple at basic pH.
- Buffer: Maintaining a balanced pH in the media.
Additional resources
Cell Proliferation AssaysAssay development servicesBerg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan. Freshney, R. I. (2005). Serum?Free Media. Culture of animal cells: a manual of basic technique.van der Valk, J., Bieback, K., Buta, C., Cochrane, B., Dirks, W., Fu, J., ... & Pistollato, F. (2018). Fetal bovine serum (FBS): past–present–future. Altex, 35(1), 1-20.