What does 7-AAD bind to? | AAT Bioquest

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What does 7-AAD bind to?

Posted December 20, 2021

Cell Viability Cell Cycle Assays Cell Cytotoxicity Physiological Probes

Answer

7-AAD is a membrane impermeant intercalating dye. It binds to dsDNA by intercalating between base pairs in G-C-rich regions of DNA.

Additional resources

Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

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