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AAT Bioquest

What factors will affect the molar extinction coefficient value?

Posted November 2, 2020


Answer

The molar extinction coefficient value can be determined by using the equation

ε = A/Lc where:

  • ε is the molar extinction coefficient
  • A is the amount of light absorbed by a substance for a specific wavelength
  • L is the path length
  • c is the amount of concentration of the solution

From the equation it can be seen that there are three factors that will affect the molar extinction coefficient value. The three factors include:

  1. The amount of light absorbed by the substance for a specific wavelength
  2. The distance that the light travels through the solution
  3. The concentration of the absorbing solution per unit volume
Additional resources

Factors affecting the measurement of the extinction coefficient of Fe3+ ions in a Fricke dosimeter solution

Extinction Coefficient Finder