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What is the difference between staining buffer and FACS buffer?
Posted April 24, 2020
Answer
Formulations of FACS buffer generally include around 2-5% FBS or 1% BSA in PBS. FACS buffers may also include sodium azide (0.05-0.1%) and EDTA (~1 mM). This is used as the staining buffer in FACS, as well as for washing.
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