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What is the best blocking buffer for immunofluorescence ?
Posted April 17, 2020
Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)PBSbufferimmunofluorescence
Answer

For immunofluorescence, the best blocking buffer to use is 1% BSA in PBS. Alternatively, a 5% serum from the secondary antibody’s host is also effective.

Additional resources
Secondary AntibodiesCommonly used biological buffers.

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