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AAT Bioquest

When should DTT be added in tissue lysis?

Posted April 17, 2020


Answer

Dithiothreitol (DTT) is a reducing agent that reduces disulfide bonds and protects from oxidation damage. DTT can be added to lysis buffer when the protein of interest in the experiment is redox-sensitive but should be left out in other cases since it has the potential to cause loss of structure and activity as a result of the broken disulfide bonds.

Additional resources

ReadiUse™ mammalian cell lysis buffer *5X*