Why are my cells leaking Fluo-4 AM?
Posted February 19, 2020
Fluo-4 AM typically has reasonable cell retention-if you are experiencing unexpected leakage of the indicator, we recommend initial troubleshooting as follows:
- Check that your protocol is optimized for the selected cell type.
- Lower temperatures will mitigate cell leakage, or adding probenicid to the media. Both can negatively effect results, however, so employ either method with caution.