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Are there any calcium indicators that don't require probenecid (PBC)?
Posted April 1, 2020
Calcium dyescalcium fluxcalcium indicatorfluorescent calcium indicatorno probenecid
Answer

Yes, the following calcium indicators have improved intracellular retention properties and do not require the use of probenecid:

Note: The aformentioned dyes require much less concentrations 0.2 to 1 mM, compared to Fluo-3 AM and Fluo-4 AM that require concentrations of 2.5 mM.

Additional resources
Calbryte™ 520 AM

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