AAT Bioquest

Are coumarin dyes pH sensitive?

Posted October 14, 2022


Coumarin dyes such as AMCA and 7-aminocoumarin are both insensitive to pH. However, there are some coumarin derivatives that show varying fluorescent colors in acidic and alkaline environments; the fluorescence changed from blue to yellow-green when the pH of the solution changed from acidic to alkaline. 

Additional resources


AMCA Alkyne

Novel coumarin-based fluorescent pH indicators, probes and membranes covering a broad pH range