To optimize protein desalting, consider the following factors:
Timing of Desalting: Determine when to desalt your protein sample or change the buffer. This step is crucial when salt might interfere with subsequent processes or when the sample requires a different buffer. Desalting can be performed before, during, or after purification, or before analysis.
Consider the following instances for performing desalting:
Sample Loading: The sample-to-resin volume ratio is critical for effective desalting. To minimize dilution while maintaining good separation, it's recommended not to exceed a sample volume of 30% of the total bed volume.
Choose the Right Column: Consider factors such as the molecular weight of your protein, the volume of your sample, desired throughput, and the equipment or technique you plan to use when selecting a prepacked column or plate.