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What is the purpose of dialysis in protein isolation?
Posted April 19, 2024
Chemical Reagents
Buffer Preparations and Recipes
affinity purification
cell lysis
detergent
Answer
The primary purpose of
dialysis
in protein isolation is to remove small, unwanted compounds from macromolecules in solution. In the laboratory, it is widely used for removing salts, preservatives, labeling reagents and reducing agents in a solution.
Additional resources
Dialysis and concentration of protein solutions
Cell Lysis, Dialysis & Desalting Reagents
ReadiUse™ mammalian cell lysis buffer *5X*
ReadiUse™ Disposable PD-10 Desalting Column
Detergents
Buffer Preparations and Recipes
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