What is a typical dialysis procedure

A typical dialysis procedure for protein samples typically involves the following steps: 

1. Prepare or pre-wet the membrane as per the instructions provided. 
2. Load the sample into the dialysis tubing or device.
3. Dialyze the sample for about one to two hours at room temperature.
4. Replace the dialysis buffer and continue dialyzing for another one to two hours. 
5. Replace the dialysis buffer once more and dialyze overnight at a temperature of 4°C.

Note: For optimal outcomes, ensure that the dialysate (dialysis buffer) volume is at least 200 times greater than the sample volume.