What is a typical dialysis procedure
Posted April 19, 2024
Answer
A typical dialysis procedure for protein samples typically involves the following steps:
- Prepare or pre-wet the membrane as per the instructions provided.
- Load the sample into the dialysis tubing or device.
- Dialyze the sample for about one to two hours at room temperature.
- Replace the dialysis buffer and continue dialyzing for another one to two hours.
- Replace the dialysis buffer once more and dialyze overnight at a temperature of 4°C.
Note: For optimal outcomes, ensure that the dialysate (dialysis buffer) volume is at least 200 times greater than the sample volume.