AAT Bioquest

Is DAPI soluble in PBS?

Posted November 16, 2022


DAPI is not very soluble in PBS, even after the addition of heat and sonication. It dissolves better in water than in any buffer. It will not dissolve in PBS because it has a ph of 7.4, while water’s ph is 7.  It’s important to add that because DAPI is hydrophobic, it still has a hard time dissolving in water. You would have to  sonicate the DAPI for it to dissolve.

Additional resources

DAPI - Blue Fluorescent DNA Stain

Nuclear Violet™ LCS1 *5 mM DMSO Solution*

DAPI as a useful stain for nuclear quantitation