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AAT Bioquest

What are the advantages of nested PCR?

Posted February 28, 2024


Answer

There are various advantages of Nested PCR. By using two sets of primers in two consecutive PCR reactions, nested PCR significantly reduces nonspecific amplification, leading to higher specificity for the target sequence. Nested PCR can also amplify target sequences present in low abundance. This is because the second PCR reaction ensures that the amplicons from the first reaction predominantly contain the target sequence and its neighboring sequences. It is also efficient for amplifying segments of long templates.  

Additional resources

Nested Polymerase Chain Reaction (PCR)

Advantages & Limits of Nested PCR vs. Standard PCR

Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*