What are the advantages of nested PCR?

There are various advantages of Nested PCR. By using two sets of primers in two consecutive PCR reactions, nested PCR significantly reduces nonspecific amplification, leading to higher specificity for the target sequence. Nested PCR can also amplify target sequences present in low abundance. This is because the second PCR reaction ensures that the amplicons from the first reaction predominantly contain the target sequence and its neighboring sequences. It is also efficient for amplifying segments of long templates.