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What are the advantages of single step RT-qPCR?
Posted June 19, 2024
Answer
One advantage of single step RT-qPCR is that it requires minimal sample manipulation, which reduces the potential for error and decreases the time to do the experiment. It also involves using closed-tube reactions, thus lowering the risk of pipetting mistakes and sample contamination.
Additional resources
One-step vs. two-step RT-qPCR—tips for choosing the right protocol