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What are the differences between endpoint PCR and qPCR?
Posted June 19, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)
Answer
Basis of differentiation
Endpoint PCR
qPCR
Detection
End-point detection of PCR products after amplification cycle
Continuous monitoring of PCR amplification in real-time
Dynamic Range
Limited dynamic range
Wide dynamic range
Sensitivity
Comparatively lower sensitivity
Comparatively higher sensitivity 
Speed
Has a longer turnaround time 
Faster results due to real-time monitoring of amplification
Automation
Has less automation 
Has a higher degree of automation, and is higher throughput
Additional resources
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and EverythingReal-Time PCR (qPCR) Helixyte™ Green *20X Aqueous PCR Solution* DNA Concentration Calculator

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