What are the differences between endpoint PCR and qPCR?
Posted June 19, 2024
Answer
Basis of differentiation |
Endpoint PCR |
qPCR |
Detection |
End-point detection of PCR products after amplification cycle |
Continuous monitoring of PCR amplification in real-time |
Dynamic Range |
Limited dynamic range |
Wide dynamic range |
Sensitivity |
Comparatively lower sensitivity |
Comparatively higher sensitivity |
Speed |
Has a longer turnaround time |
Faster results due to real-time monitoring of amplification |
Automation |
Has less automation |
Has a higher degree of automation, and is higher throughput |
Additional resources
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything