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What are the differences between endpoint PCR and qPCR?
Posted June 19, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)
Answer

Basis of differentiation

Endpoint PCR

qPCR

Detection

End-point detection of PCR products after amplification cycle

Continuous monitoring of PCR amplification in real-time

Dynamic Range

Limited dynamic range

Wide dynamic range

Sensitivity

Comparatively lower sensitivity

Comparatively higher sensitivity 

Speed

Has a longer turnaround time 

Faster results due to real-time monitoring of amplification

Automation

Has less automation 

Has a higher degree of automation, and is higher throughput

Additional resources
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and EverythingReal-Time PCR (qPCR) Helixyte™ Green *20X Aqueous PCR Solution* DNA Concentration Calculator

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