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AAT Bioquest

What are the differences between endpoint PCR and qPCR?

Posted June 19, 2024


Answer

Basis of differentiation

Endpoint PCR

qPCR

Detection

End-point detection of PCR products after amplification cycle

Continuous monitoring of PCR amplification in real-time

Dynamic Range

Limited dynamic range

Wide dynamic range

Sensitivity

Comparatively lower sensitivity

Comparatively higher sensitivity 

Speed

Has a longer turnaround time 

Faster results due to real-time monitoring of amplification

Automation

Has less automation 

Has a higher degree of automation, and is higher throughput

Additional resources

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

Real-Time PCR (qPCR)

Helixyte™ Green *20X Aqueous PCR Solution*

DNA Concentration Calculator