What types of fluorescent reporters are used in real-time quantitative PCR (qPCR)?
Posted June 19, 2024
Answer
In qPCR assays, two common types of fluorescent reporters are used: double-stranded DNA binding dyes and labeled primers or probes (e.g. molecular beacon probes).
Additional resources
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology