logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
What types of fluorescent reporters are used in real-time quantitative PCR (qPCR)?
Posted June 19, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)
Answer

In qPCR assays, two common types of fluorescent reporters are used: double-stranded DNA binding dyes and labeled primers or probes (e.g. molecular beacon probes). 

Additional resources
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecologyReal-Time PCR (qPCR) Helixyte™ Green *20X Aqueous PCR Solution* DNA Concentration Calculator

What should I choose for my experiment, PCR or qPCR? What are the disadvantages of fluorescent reporters used in qPCR?What are the similarities between endpoint PCR and qPCR?What are the advantages of End-point PCR?What are the advantages of 2-step RT-qPCR?