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AAT Bioquest

What are the advantages of End-point PCR?

Posted June 19, 2024


Answer

One advantage of end-point PCR is that their protocols are straightforward to design and carry out, requiring minimal optimization. Additionally, endpoint PCR typically requires fewer reagents compared to real-time PCR techniques. Another pro is that there are only basic steps involved such as setting up PCR reactions, running the amplification cycles, and analyzing the results. This means individuals without extensive training can perform endpoint PCR assays effectively.

Additional resources

Research Techniques Made Simple: Polymerase Chain Reaction (PCR)

Real-Time PCR (qPCR)

Helixyte™ Green *20X Aqueous PCR Solution*

DNA Concentration Calculator