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What are the disadvantages of 2-step RT-qPCR?
Posted June 19, 2024
Answer
The main limitation of RT-qPCR is the need for an additional open-tube step, which involves more pipetting and longer labor-time. Thus, this increases the potential for contamination and variability, making two-step RT-qPCR less suitable for high-throughput applications.
Additional resources
One-step vs. two-step RT-qPCR—tips for choosing the right protocol