Third generation sequencing technologies offer significant advantages over other methods by producing longer reads (1500-100,000 base pairs). Third generation sequencing technologies offer additional benefits, such as faster sequencing and portability. Compared to second generation sequencing, they require minimal sample preparation, allowing for the design of smaller, more portable equipment. A third advantage is third generation sequencing could potentially detect epigenetic markers directly, as they produce a unique signal different from the four nucleotide bases, simplifying the detection process.