What are the disadvantages of third generation NGS?

One main disadvantage involves the accurate identification of nucleotide bases. Error rates remain notably higher compared to second generation sequencing, largely due to the instability of the molecular machinery involved. Another drawback is the rapid pace of sequencing can cause signals from adjacent bases to blur, complicating the interpretation of signals and the analyzing of sequences. A third limitation is that since humans share 99.9% of their genes on average, with only about one differing base per every thousand bases between individuals, the elevated error rates associated with third generation sequencing pose a significant obstacle. These errors hinder the accurate classification of individual genetic differences within the same species.