AAT Bioquest

What are the applications of cell sorting?

Posted May 24, 2023


Cell sorting is a process that isolates one or more target cell populations from a heterogeneous biological sample. The resulting homologous cell populations have significant applications in research and as therapeutics. 

Applications of cell sorting include but are not limited to: 

  • Isolating WBC from tissue
  • Isolating T cells, B cells, and other immune cells from peripheral blood
  • Genetically modifying and expanding a specific target cell type for cell therapy research applications such as T cell therapy research or disease modeling
  • Fusing enriched plasma cells with myeloma cells to create hybridomas 
  • Capturing CTCs or circulating tumor cells from blood
  • Increasing the sensitivity of analytical techniques such as cell isolation for FISH analysis or cell isolation of HLA analysis
  • Preparing a sample of blood separated from plasma
  • Conducting molecular analysis of a single cell type, including epigenetic analysis and RNA expression
  • Studying the in vitro effects of potential drug candidates on specific cell populations
  • Separating pathogenic bacteria from food
  • Using purified cells for adoptive cell transfer experiments directly in different animal models
Additional resources

Large-Volume Microfluidic Cell Sorting for Biomedical Applications

Fluorescence Activated Cell Sorting (FACS)

ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*