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AAT Bioquest

What are the applications of Reduced-Representation Sequencing (RRS)?

Posted April 18, 2024


Answer

One application of RRS is generating molecular markers. This involves identifying individual genetic variations such as single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels), incorporating them into population-wide markers, and using them to calculate genetic distances. Another application is helping in de novo genome assembly. This task involves aiding in the assembly of draft genomes by annotating genes. A third application is genetic map construction and QTL mapping. This involves organizing genetic markers into linkage groups and identifying regions associated with quantitative trait loci (QTL) based on observed traits. RRS is also involved in population genetic evolution. Using SNP data, this analysis examines evolutionary patterns, gene flow, and relatedness among individuals within a population. Lastly, by sequencing and analyzing the entire genome of a population, RRS identifies genetic variants associated with specific traits or phenotypes.

Additional resources

A restriction enzyme reduced representation sequencing approach for low-cost, high-throughput metagenome profiling

Next Generation Sequencing (NGS)

MagaDye™ 535-ddGTP