What are the components required to set up the chain termination PCR? | AAT Bioquest

Search by catalog number, product name, application...

Products

Technologies

Applications

Services

Resources

Selection Guides

About

What are the components required to set up the chain termination PCR?

Posted February 20, 2024

DNA and RNA Quantitation DNA sequencing Nucleic Acids Nucleus Polymerase Chain Reaction (PCR)Sanger Sequencing

Answer

The components required to assemble chain termination PCR are:

Sequencing primer
DNA template
Dideoxynucleotides
Buffer
DNA polymerase

Additional resources

Sanger Sequencing Polymerase Chain Reaction (PCR) Gelite™ Safe DNA Gel Stain *10,000X Water Solution*

What does each component in chain termination PCR do?What are the different techniques commonly used to amplify DNA fragments before sequencing?What are the advantages of SMRT sequencing?What are features of next generation sequencing?What are the disadvantages of SMRT sequencing?