Basis of differentiation | FISH | ISH |
Definition | Is a technique which uses fluorescently labeled nucleic acid probes to bind to specific target sequences within cells or tissue samples | In situ hybridization involves using a non-fluorescent hapten labeled strand of DNA, RNA, or modified nucleic acids to detect sequences within intact cells or preserved tissue sections |
Use of fluorescent probes | Uses fluorescent probes | Does not involve fluorescent probes |
Sensitivity | High sensitivity due to fluorescent detection | Is comparatively less sensitive |
Speed | Rapid visualization of results | Takes comparatively longer than FISH due to extra steps |
Multiplexing | Capable of multiplexing with different fluorophores | Exhibits less multiplexing capability, typically single color detection |
Detection method | Autoradiography or immunohistochemistry |