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AAT Bioquest

What are the disadvantages of using dsDNA probes for performing in situ hybridization?

Posted March 27, 2024


Answer

One disadvantage is that they can self-hybridize, forming secondary structures such as hairpins or loops due to intramolecular base pairing. dsDNA probes also typically require denaturation before hybridization and are less sensitive for target detection. 

Additional resources

Technical Review: In Situ Hybridization

Fluorescent in situ hybridization (FISH)

ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit