What are the disadvantages of using RNA probes for performing in situ hybridization? | AAT Bioquest

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What are the disadvantages of using RNA probes for performing in situ hybridization?

Posted March 27, 2024

DNA and RNA Quantitation FISH Fluorescence Nucleic Acids Nucleus Physiological Probes in situ hybridization

Answer

The key disadvantage is that sensitivity of RNA probes to RNases requires strict experimental controls to prevent unintended RNA degradation. Proper handling of samples and instruments is essential to minimize the risk of RNase contamination.

Additional resources

Technical Review: In Situ Hybridization Fluorescent in situ hybridization (FISH) ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

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