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What are the disadvantages of using synthetic oligonucleotide probes for performing in situ hybridization?
Posted March 27, 2024
DNA and RNA QuantitationFISHFluorescenceNucleic AcidsNucleusPhysiological Probesin situ hybridization
Answer

The key limitation is that synthetic probes rely on the accuracy of the nucleotide sequence information provided.

Additional resources
Technical Review: In Situ HybridizationFluorescent in situ hybridization (FISH) ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

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