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AAT Bioquest

What are the differences between introns and exons?

Posted March 14, 2024


Answer

Basis of differentiation

Introns

Exons

Definition

Are noncoding segments of DNA and RNA molecules which are spliced out during the pre-mRNA stage of transcription

Are coding segments of an RNA transcript, or the DNA encoding it, which are translated into proteins

Cell type

Is found in eukaryotes

Is found in eukaryotes and prokaryotes

Conservation

Typically less conserved across species

Typically more conserved across species

DNA bases

DNA bases are located in between exons

DNA bases are translated into proteins

Mechanism

Introns are spliced in the nucleus prior to the mRNA entering the cytoplasm

Once mRNA has been processed in the nucleus, it retains the exons

Length

Are typically much longer than exons

Are typically much shorter than introns

Additional resources

Difference Between Introns and Exons

DNA and RNA Quantitation

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

Helixyte™ Green *10,000X Aqueous PCR Solution*