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What are the disadvantages of fluorescent reporters used in qPCR?
Posted June 19, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)
Answer
One disadvantage of dyes is that since the signal isn't sequence-specific, it requires additional analysis like melt curve analysis for confirmation. The main cons of these probes is that they have complex probe designs and are costly.