What are the disadvantages of fluorescent reporters used in qPCR?
Posted June 19, 2024
Answer
One disadvantage of dyes is that since the signal isn't sequence-specific, it requires additional analysis like melt curve analysis for confirmation. The main cons of these probes is that they have complex probe designs and are costly.
Additional resources
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology