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What are the disadvantages of RAD?
Posted April 18, 2024
DNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusSanger Sequencing
Answer
One drawback is that RAD still has a comparatively higher cost per individual than microsatellites. It also has a more sophisticated experimental protocol than other methods. 
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Optimized double-digest genotyping by sequencing (ddGBS) method with high-density SNP markers and high genotyping accuracy for chickensSanger Sequencing MagaDye™ 535-ddGTP

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