There are various limitations of the spread plate method. 

• An additional instrument is required, such as a spreader, for even distribution. 
• For solid or semisolid samples, they must be converted into suspensions before inoculation. 
• It requires liquid or easily soluble samples, which often need serial dilution. 
• It may not adequately support the growth of certain microorganisms like anaerobes and micropiles. 
• Sterilization of tools is also required after each plate to avoid cross-contamination. 
• This method also requires pre-solidified specific media beforehand, and thus prior knowledge is necessary about potential microorganisms in the sample.