The general procedure of the streak plate method is described in the steps below. 

1. Sterilize the inoculating loop by placing it in the bunsen burner flame until it turns red hot. Allow it to cool down.
2. Using the cooled loop, choose an isolated colony from the agar plate culture and spread it over one-fourth of the plate by making parallel streaks.
3. Sterilize the loop again in the flame
4. Rotate the plate 90°, and lightly pass the loop through the previously inoculated area 1-2 times. Then streak into the next quadrant without overlapping the previous streaks.
5. Sterilize the loop in the flame once more.
6. Rotate the plate again by 90° and lightly pass the loop through the previously inoculated area 1-2 times. Then streak into the next quadrant without overlapping the previous streaks.
7. Sterilize the loop again.
8. Repeat the previous step, streaking the remaining portion of the plate
9. Invert the plate and place it in an incubator set at 37°C for 24 hours.