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AAT Bioquest

What are the tips for the best results when performing the streak plate method?

Posted January 9, 2024


Answer

There are several important tips one should consider when performing the streak plate method. One suggestion is to use only a small amount of inoculum, since that is all that is required. Another tip is to gently streak the plate to avoid digging into the agar. Additionally, one should flame the loop after each quadrant is streaked for sterilization. Another tip is to ensure the plate surface is dry without any water droplets to prevent contamination. An additional suggestion is to label plates before streaking, to avoid confusion in identification. One should also make sure to maintain enough distance when using the plate to avoid respirating on it. This is also to prevent contamination. Lastly, the edge of the plate should not be touched when streaking or swabbing the agar.  

Additional resources

Streak Plate Technique

Cell Sample Preparation

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