What are the limitations of the streak plate method?

There are also various limitations of the streak plate method. One drawback is that it lacks the ability to quantify microbial load. Another limitation is it might not be suitable for large samples with a high viable count, resulting in non-isolated colonies. It also favors aerobic organisms over anaerobes and cannot purify syntrophic bacteria. It also requires skill to avoid damaging the agar surface during streaking, and is also time-consuming as it requires an inoculating loop as an additional tool. There is also a higher risk of contamination due to frequent lid opening and loop use.