logo
AAT Bioquest

What are the similarities between endpoint PCR and qPCR?

Posted June 19, 2024


Answer

Both endpoint PCR and qPCR rely on the same basic principle of DNA amplification using DNA polymerase enzymes and specific primers for the target DNA sequence. They also both involve thermal cycling, including denaturation, annealing, and extension steps to amplify the target DNA. 

Additional resources

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

Real-Time PCR (qPCR)

Helixyte™ Green *20X Aqueous PCR Solution*

DNA Concentration Calculator