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AAT Bioquest

What are the types of cell labeling?

Posted January 16, 2024


Answer

Most common types of cell labeling include: 

  • Fluorescence Microscopy: Fluorescence microscopy involves the use of optical filters to separate emitted light from excited light. This facilitates the simultaneous observation of different biomolecules, enabling the precise identification of cells and cellular components.
  • Fluorescence In Situ Hybridization (FISH): FISH is employed to localize specific DNA sequences on chromosomes. In this process, target DNA sequences are hybridized and identified using fluorescent DNA or RNA probes. FISH is widely used for mapping genes on chromosomes and for detecting chromosomal abnormalities. 
  • Flow Cytometry: Flow cytometry involves passing cells and particles through a light beam of detectors that can analyze the labeled antibodies. This process enables researchers to isolate and characterize different cell types and also measure their size and volume. Flow cytometry is widely used in clinical practice. 
  • Fluorescence Correlation Spectroscopy (FCS): FCS is widely used to analyze changes in fluorochromes, which may be brought about by physical, chemical, biological factors.
  • Microarrays: Microarrays are useful for creating comprehensive gene expression profiles from data gathered by studying gene expression of many genes simultaneously. 
Additional resources

Nanoparticles for cell labeling

Fluorescence Microscopy

Fluorescent in situ hybridization (FISH)

ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit