Fixatives may be classified into three types based on their chemical composition, type of structures fixed, and number of structures fixed.
Based on chemical composition, fixatives may be classified as:
- Physical agents – Heat and microwaves
- Oxidizing agents – Osmium tetroxide
- Aldehydes – Formaldehyde, glutaraldehyde, acrolein
- Coagulants – Acetic acid, ethyl alcohol, methyl alcohol,
- Miscellaneous – Mercuric acid, picric acid
Based on types of structures fixed, fixatives may be classified as:
- Cytologic fixatives – Clarke’s fluid, Flemming’s fluid, glacial acetic acid
- Histochemical fixatives – Formaldehyde, glutaraldehyde, vapor fixatives
- Microanatomical fixatives – Helly’s fluid, Bouin’s fluid, Heidenhain’s fluid,
Based on number of structures fixed, fixatives may be classified as:
- Simple fixatives – Formaldehyde, picric acid, osmium tetroxide
- Compound fixatives – Zenker’s fluid, Bouin’s fluid, formol saline