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What facilitates the attachment of adaptors to the DNA fragment?
Posted April 18, 2024
DNA and RNA QuantitationDNA FragmentationGene Expression Analysis & GenotypingNucleic AcidsNucleus
Answer

Initially, the DNA fragment ends are made blunt and the 5' ends are phosphorylated using a combination of enzymes. Taq polymerase then assists in attaching adapters to the DNA fragments. It's crucial to maintain an optimal ratio of 10 adapters to one fragment to prevent the formation of adapter dimers. 

Additional resources
Adapter dimers causes, effects, and how to remove themDNA Fragmentation Gelite™ Green Nucleic Acid Gel Staining Kit

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