AAT Bioquest

What is TE (Tris-EDTA) buffer?

Posted September 29, 2022


TE (Tris-EDTA) buffer is widely used as a storage or dilution buffer solution in molecular biology, especially in procedures involving DNA, RNA or cDNA. The primary function of the Tris-EDTA buffer is to solubilize nucleic acids while protecting them from enzymatic lysis. TE is an abbreviation of the components that make up the buffer solution. ‘T’ stands for Tris, a pH buffer that is used in many laboratory processes to maintain the pH of a solution. ‘E’ stands for EDTA, or ethylenediamine tetra-acetic acid, a divalent metal ion chelating ion. In DNA or RNA extraction procedures, EDTA reduces the activities of nucleases that digest the nucleic acids. This protects the DNA and RNA from degradation. 

Additional resources

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