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What is the composition of TE buffer?
Posted October 3, 2022
Answer
TE (Tris-EDTA) buffer is composed of two reagents – Tris and EDTA. Tris is a commonly used pH buffer. It has a pH buffer range of 7.5-8.5. Nuclease activity is low in this environment. EDTA (ethylenediamine tetra-acetic acid) is a divalent metal chelating agent. It chelates metal ions necessary for nucleases to function, thus further inactivating the nuclease and protecting nucleic acids from degradation.
Additional resources
Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe