AAT Bioquest

What is the composition of TE buffer?

Posted October 3, 2022


TE (Tris-EDTA) buffer is composed of two reagents – Tris and EDTA. Tris is a commonly used pH buffer. It has a pH buffer range of 7.5-8.5. Nuclease activity is low in this environment. EDTA (ethylenediamine tetra-acetic acid) is a divalent metal chelating agent. It chelates metal ions necessary for nucleases to function, thus further inactivating the nuclease and protecting nucleic acids from degradation. 

Additional resources

Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe

EDTA Solution Preparation and Recipe

In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris-EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis