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Why is Tris used in the TE buffer?
Posted October 4, 2022
Answer
Tris is a widely used pH buffer. It is used in the TE buffer to maintain the pH of the DNA and increase the permeability of the cell wall. Tris is a (hydroxymethyl)aminomethane. Its molecular formula is (HOCH2)3CNH2.
Additional resources
Conformational change of bovine serum albumin induced by O.IO m Tris-EDTA-boric acid buffer at pH9
Conformational change of bovine serum albumin induced by O.IO m Tris-EDTA-boric acid buffer at pH9