AAT Bioquest

What is the principle of alamar Blue assay?

Posted June 21, 2023


The alamar Blue assay uses a nontoxic, cell-permeable dye, resazurin, which serves as a REDOX indicator dye. The alamar Blue assay relies on the conversion of the initially weakly fluorescent blue dye resazurin to a highly fluorescent pink compound called resorufin in response to cellular metabolic activity. The intensity of the pink fluorescence is directly linked to the quantity of viable cells, providing a direct measure of cell health.

Additional resources

Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays

Cell Viability Assays

Resazurin, sodium salt *CAS 62758-13-8*